Rat Low Density Lipoprotein Receptor (LDLR) ELISA Kit
The Rat Low Density Lipoprotein Receptor (LDLR) ELISA Kit is a reliable tool for the accurate quantification of LDLR levels in rat serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, providing reproducible results for a variety of research applications in the field of cardiovascular and lipid metabolism research.The LDLR is a key protein involved in regulating cholesterol homeostasis by mediating the uptake of LDL particles into cells. Dysregulation of LDLR expression is associated with various metabolic disorders, including atherosclerosis, hypercholesterolemia, and familial hypercholesterolemia.
Therefore, the LDLR ELISA Kit serves as a valuable tool for studying the role of LDLR in these conditions and developing novel therapeutic strategies.Overall, the Rat Low Density Lipoprotein Receptor (LDLR) ELISA Kit offers researchers a reliable and efficient method for quantifying LDLR levels in rat samples, facilitating in-depth investigations into the molecular mechanisms underlying cardiovascular diseases and lipid metabolism disorders.
Product Name:
Rat Low Density Lipoprotein Receptor (LDLR) ELISA Kit
Product Code:
RTDL00625
Size:
96 Assays
Target:
LDLR
Synonyms:
FH, FHC, Familial Hypercholesterolemia
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
