Rat Membrane Protein, Palmitoylated 2 (MPP2) ELISA Kit
The Rat Membrane Protein Palmitoylated 2 (MPP2) ELISA Kit is a powerful tool for the quantitative detection of MPP2 levels in rat samples. This kit offers exceptional sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.MPP2 is a key membrane protein involved in cellular processes such as cell signaling, cell adhesion, and membrane trafficking. It plays a crucial role in modulating cell-cell interactions and regulating intracellular signaling pathways.
Understanding the expression and function of MPP2 is essential for investigating its role in various physiological and pathological conditions.Whether studying signal transduction pathways, investigating protein interactions, or exploring membrane dynamics, the Rat MPP2 ELISA Kit is an invaluable resource for researchers seeking to elucidate the role of MPP2 in cellular biology.'s physiology.
Product Name:
Rat Membrane Protein, Palmitoylated 2 (MPP2) ELISA Kit
Product Code:
RTDL00689
Size:
96 Assays
Target:
MPP2
Synonyms:
DLG2, MAGUK P55 Subfamily Member 2, Discs Large,Homolog 2
Detection Method:
Sandwich
Range:
0.625-40U/L
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
