The Rat Neuron-Specific Enolase (NSE) ELISA Kit is a state-of-the-art assay meticulously designed to quantitatively detect NSE levels in various rat biological samples. Neuron-Specific Enolase, a key neuronal marker, serves as a diagnostic biomarker for neuronal injury or disease, making it a valuable indicator for neuro-related research and clinical applications. This cutting-edge ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results that are crucial for understanding neuronal health and function. Manufactured under stringent quality control standards, this kit delivers robust performance, making it an invaluable tool for investigating neuronal processes and disorders. Easy-to-use and reliable, this ELISA kit is an excellent choice for researchers seeking high-quality data in the field of neuroscience.
Product Name:
Rat NSE (Neuron Specific Enolase) ELISA Kit
SKU:
AEES00310
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
