The Rat Pentraxin 3 Long (PTX3) ELISA Kit is a reliable and accurate tool for the detection of PTX3 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Pentraxin 3 Long (PTX3) is a key protein involved in the immune response, inflammation, and tissue repair processes. It plays a vital role in various pathological conditions, including infectious diseases, autoimmune disorders, and cardiovascular diseases.
The measurement of PTX3 levels can provide valuable insights into disease progression and potential therapeutic interventions.With its robust performance and user-friendly design, the Rat Pentraxin 3 Long (PTX3) ELISA Kit is an essential tool for researchers studying the role of PTX3 in health and disease. Trust in this kit for accurate and reliable results to further your research endeavors.
Product Name:
Rat Pentraxin 3, Long (PTX3) ELISA Kit
Product Code:
RTDL00885
Size:
96 Assays
Target:
PTX3
Synonyms:
TNFAIP5, TSG-14, Pentaxin-Related Gene,Rapidly Induced By IL-1 Beta, Tumor Necrosis Factor Alpha-Induced Protein 5, Tumor necrosis factor-inducible gene 14 protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
