The Rat Platelet Glycoprotein IIb/IIIa ELISA Kit is a powerful tool for researchers looking to accurately detect levels of this important protein in rat samples. Glycoprotein IIb/IIIa is a key player in platelet function, crucial for blood clotting and wound healing processes. This ELISA kit boasts high sensitivity and specificity, providing researchers with reliable and reproducible results for a variety of research applications. Whether studying thrombosis, hemostasis, or other platelet-related conditions, this kit offers the precision and accuracy needed for successful experimentation. By quantifying levels of Platelet Glycoprotein IIb/IIIa, researchers can gain valuable insights into the mechanisms underlying clot formation and vascular health.
This information is essential for understanding and potentially developing new therapies for conditions such as cardiovascular disease and thrombotic disorders. Overall, the Rat Platelet Glycoprotein IIb/IIIa ELISA Kit is an essential tool for researchers studying platelet function and its role in health and disease in rat models. Trust in the reliability and accuracy of this kit to advance your research and discovery in this important area of study.
Product Name:
Rat Platelet Glycoprotein IIb + IIIa ELISA Kit
SKU:
RTFI00828
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.375 ng/mL
Range:
0.625-40 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
94-104%
82-101%
85-97%
EDTA Plasma (n = 5)
85-104%
82-92%
80-92%
Heparin Plasma (n = 5)
85-101%
85-96%
82-96%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
92-96
93
EDTA Plasma (n = 5)
85-101
94
Heparin Plasma (n = 5)
86-104
99
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
