The Rat Protein Kinase C Beta-1 (PKCB1) ELISA Kit is specifically developed for the precise measurement of PKCB1 levels in rat samples such as serum, plasma, and cell lysates. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an essential tool for various research applications.Protein Kinase C Beta-1 is a key enzyme involved in signal transduction pathways, regulating various cellular processes such as cell growth, differentiation, and apoptosis.
Dysregulation of PKCB1 has been linked to multiple diseases including cancer, diabetes, and cardiovascular disorders, highlighting its importance as a potential therapeutic target and biomarker.With the Rat PKCB1 ELISA Kit, researchers can efficiently study the role of PKCB1 in disease progression, evaluate drug efficacy, and explore potential treatment strategies. Trust in the reliability and precision of this kit to advance your research and deepen your understanding of PKCB1 biology.
Product Name:
Rat Protein Kinase C Beta 1 (PKCb1) ELISA Kit
Product Code:
RTDL00838
Size:
96 Assays
Target:
PKCb1
Synonyms:
PKC-B1, PRKCB, PRKCB1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
