Rat Receptor Interacting Serine Threonine Kinase 3 (RIPK3) ELISA Kit
The Rat Receptor Interacting Serine/Threonine Kinase 3 (RIPK3) ELISA Kit is a reliable and accurate tool for detecting RIPK3 levels in rat samples, including serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides dependable and reproducible results for a variety of research applications.RIPK3 is an important signaling molecule involved in inflammation, necroptosis, and immune responses. Dysregulation of RIPK3 has been linked to various diseases, including inflammatory disorders, autoimmune diseases, and neurodegenerative conditions.
Therefore, studying RIPK3 levels can provide valuable insights into these disease processes and potential therapeutic targets.Overall, the Rat RIPK3 ELISA Kit is a valuable resource for researchers seeking to investigate the role of RIPK3 in disease pathogenesis and explore novel treatment strategies.
Product Name:
Rat Receptor Interacting Serine Threonine Kinase 3 (RIPK3) ELISA Kit
Product Code:
RTDL00906
Size:
96 Assays
Target:
RIIPK3
Synonyms:
RIP3, RIP-like protein kinase 3, Receptor-interacting protein 3
Detection Method:
Sandwich
Range:
31.2-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
