The Rat SLCO1A1 ELISA Kit is a cutting-edge tool designed for the precise measurement of SLCO1A1 levels in rat serum, plasma, and tissue lysates. With its exceptional sensitivity and specificity, this kit offers dependable and consistent results, making it an invaluable resource for a variety of research endeavors. SLCO1A1, also known as OATP1A1, is a key transporter protein involved in the uptake of endogenous and exogenous compounds, such as drugs and toxins, into cells.
Dysregulation of SLCO1A1 has been implicated in various diseases, including liver disorders and cancer, highlighting its importance as a potential therapeutic target and biomarker. By utilizing the Rat SLCO1A1 ELISA Kit, researchers can gain insights into the role of SLCO1A1 in health and disease, paving the way for the development of novel treatment strategies and diagnostic tools. Elevate your research to new heights with the Rat SLCO1A1 ELISA Kit from Assay Genie.
Product Name:
Rat SLCO1A1 ELISA Kit
SKU:
RTFI00542
Reactivity:
Rat
Assay Type:
Competitive ELISA, Coated with Antigen
Sensitivity:
0.375 ng/mL
Range:
0.625-40 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
0
1
2
3
Serum (n = 10)
90-98%
92-102%
87-99%
EDTA Plasma (n = 10)
87-99%
84-101%
82-95%
Heparin Plasma (n = 10)
97-100%
86-95%
80-100%
Recovery:
0
1
2
Serum (n = 10)
93-103
98
EDTA Plasma (n = 10)
88-104
94
Heparin Plasma (n = 10)
91-104
99
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2
Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3
Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4
Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5
Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6
Reading: Measure absorbance at 450 nm using a microplate reader.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Cell Culture Supernatant
Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant.
Cell Lysate
Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant.
Other Sample Types
Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
