Rat Thrombin Activatable Fibrinolysis Inhibitor (TAFI) ELISA Kit
The Rat Thrombin Activatable Fibrinolysis Inhibitor (TAFI) ELISA Kit is a high-quality assay designed for the accurate measurement of TAFI levels in rat samples. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.TAFI is a key protein involved in regulating fibrinolysis, the process by which blood clots are dissolved. Dysregulation of TAFI has been implicated in a range of pathologies, including thrombosis, inflammation, and cardiovascular diseases.
Studying TAFI levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.With its advanced technology and proven reliability, the Rat TAFI ELISA Kit is an indispensable tool for researchers seeking to better understand the role of TAFI in health and disease. Trust in this kit to deliver accurate and meaningful data for your research endeavors.
Product Name:
Rat Thrombin Activatable Fibrinolysis Inhibitor (TAFI) ELISA Kit
Product Code:
RTDL01002
Size:
96 Assays
Target:
TAFI
Synonyms:
CPB2, CPU, PCPB, Carboxypeptidase B2, Plasma Carboxypeptidase B, Carboxypeptidase U
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
