Rat TPO-Ab (anti-Thyroid-Peroxidase Antibody) ELISA Kit
The Rat TPO (Thyroid Peroxidase) AB (Anti-Thyroid Peroxidase Antibody) ELISA Kit is specifically designed for the precise detection of anti-thyroid peroxidase antibody levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes. Thyroid peroxidase is a key enzyme involved in thyroid hormone synthesis, and the presence of anti-thyroid peroxidase antibodies is associated with autoimmune thyroid disorders such as Hashimoto's thyroiditis.
Monitoring these antibodies can provide valuable insights into the pathogenesis and progression of autoimmune thyroid diseases, facilitating research efforts aimed at developing effective diagnostic tools and treatments. Overall, the Rat TPO AB ELISA Kit provides researchers with a reliable tool for investigating the role of anti-thyroid peroxidase antibodies in thyroid-related conditions, ultimately contributing to advancements in the field of autoimmune disease research.
Product Name:
Rat TPO-Ab (anti-Thyroid-Peroxidase Antibody) ELISA Kit
SKU:
RTFI01361
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antigen
Sensitivity:
0.469 IU/mL
Range:
0.781-50 IU/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
90-105%
88-105%
86-100%
EDTA Plasma (n = 5)
89-100%
84-97%
83-98%
Heparin Plasma (n = 5)
81-90%
86-100%
85-97%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
88-100
94
EDTA Plasma (n = 5)
90-100
95
Heparin Plasma (n = 5)
86-105
96
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Biotin-Labeled Antigen Binding: Add biotin-labeled antigen working solution and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add SABC working solution and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes until color develops.
6
Stop Reaction & Reading: Add stop solution and read absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes and collect supernatant.
Plasma
Collect in anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue with PBS or lysis buffer, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells on ice using lysis buffer, centrifuge and collect supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antigen (Concentrated, 100X)
60 ul
120 ul
2-8°C (Avoid direct light)
HRP-Streptavidin Conjugate (SABC, 100X)
60 ul
120 ul
2-8°C (Avoid direct light)
TMB Substrate
5 ml
10 ml
2-8°C (Avoid direct light)
Sample Dilution Buffer
10 ml
20 ml
2-8°C
Antigen Dilution Buffer
5 ml
10 ml
2-8°C
SABC Dilution Buffer
5 ml
10 ml
2-8°C
Stop Solution
5 ml
10 ml
2-8°C
Wash Buffer(25X)
15 ml
30 ml
2-8°C
Plate Sealer
3 pieces
5 pieces
-
Technical Manual
1 copy
1 copy
-
Kayumov, K., et al.
The Restorative Role of Flavonoids in Immunological, Antioxidant, and Microbiota Mechanisms of Autoimmune Thyroiditis
