The Rat TSLP (Thymic Stromal Lymphopoietin) CLIA Kit is specifically designed to accurately measure TSLP levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it an invaluable tool for researchers involved in studying immune responses and inflammatory diseases in rats.TSLP is a key cytokine that plays a crucial role in regulating immune responses, particularly in the development of allergic inflammation and autoimmune diseases. By measuring TSLP levels, researchers can gain important insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the Rat TSLP CLIA Kit offers a comprehensive solution for studying TSLP biology in rat models and advancing our understanding of immune-mediated diseases.
Product Name:
Rat TSLP (Thymic Stromal Lymphopoietin) CLIA Kit
SKU:
RTES00549
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.