Rat UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) ELISA Kit
The Rat UDP Glucuronosyltransferase 1 Family Polypeptide A1 (UGT1A1) ELISA Kit is a powerful tool for researchers looking to accurately measure UGT1A1 levels in rat samples. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various experimental settings.UGT1A1 is a key enzyme involved in the detoxification and metabolism of a wide range of endogenous and exogenous compounds, including drugs, toxins, and bilirubin.
Dysregulation of UGT1A1 activity has been implicated in various liver diseases, drug interactions, and disorders affecting bilirubin clearance.By accurately quantifying UGT1A1 levels in rat samples, researchers can better understand the role of this enzyme in health and disease, paving the way for the development of therapeutic interventions and personalized medicine strategies.
Product Name:
Rat UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
