Rat Voltage Dependent Anion Channel Protein 1 (VDAC1) ELISA Kit
The Rat Voltage Dependent Anion Channel Protein 1 (VDAC1) ELISA Kit is designed for the accurate quantification of VDAC1 levels in rat samples such as serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for your research needs.VDAC1 is a key protein involved in mitochondrial function and regulation of cell death pathways. Abnormal VDAC1 expression has been linked to various diseases including cancer, neurodegenerative disorders, and metabolic conditions.
Understanding the role of VDAC1 in these diseases can provide crucial insights for therapeutic development.With the Rat VDAC1 ELISA Kit, researchers can investigate the role of VDAC1 in disease progression, evaluate potential therapeutic targets, and monitor treatment responses. This kit is a valuable tool for advancing our understanding of VDAC1 biology and its implications in various pathologies.
Product Name:
Rat Voltage Dependent Anion Channel Protein 1 (VDAC1) ELISA Kit
Product Code:
RTDL01096
Size:
96 Assays
Target:
VDAC1
Synonyms:
PORIN-31-HL, Porin 31HM, Plasmalemmal porin, Outer mitochondrial membrane protein porin 1, Voltage-dependent anion-selective channel protein 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
