Rat Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2) ELISA Kit
The Rat Zona Pellucida Glycoprotein 2 (Sperm Receptor ZP2) ELISA Kit is specifically designed for the accurate measurement of ZP2 levels in rat serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.ZP2 is a critical protein involved in sperm-egg binding and fertilization in rats. It serves as a key receptor on the zona pellucida, facilitating the interaction between sperm and egg.
Understanding the role of ZP2 is essential in reproductive biology research and can provide valuable insights into fertility, contraception, and potential treatments for infertility.Overall, the Rat Zona Pellucida Glycoprotein 2 (Sperm Receptor ZP2) ELISA Kit is an indispensable tool for researchers looking to study the intricate mechanisms of fertilization and reproductive health in rats.
Product Name:
Rat Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2) ELISA Kit
Product Code:
RTDL01121
Size:
96 Assays
Target:
ZP2
Synonyms:
ZPA, Zona Pellucida Sperm-Binding Protein 2 , Zona pellucida protein A
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
