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Anti-FIP200 Antibody (CAB14685)

SKU:
CAB14685
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy
  • Autophagy Antibodies Anti-FIP200 Antibody CAB14685
  • Autophagy Antibodies Anti-FIP200 Antibody CAB14685
  • Autophagy Antibodies Anti-FIP200 Antibody CAB14685
€139 - €419
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Description

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Antibody Name:Anti-FIP200 Antibody
Antibody SKU:CAB14685
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1500 to the C-terminus of human FIP200 (NP_055596.3).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U2OS, HeLa, Mouse testis, Rat testis
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1500 to the C-terminus of human FIP200 (NP_055596.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:DFQV GDLV LIIL DERH DNYV LFTV SPTL YFLH SESL PALD LKPG EGAS GASR RPWV LGKV MEKE YCQA KKAQ NRFK VPLG TKFY RVKA VSWN KKV
Gene ID:9821
Uniprot:Q8TDY2
Cellular Location:Cytoplasm, Nucleus, Peripheral membrane protein, Preautophagosomal structure membrane, cytosol
Calculated MW:182kDa/183kDa
Observed MW:225KDa
Synonyms:RB1CC1, ATG17, CC1, FIP200, PPP1R131
Background:The protein encoded by this gene interacts with signaling pathways to coordinately regulate cell growth, cell proliferation, apoptosis, autophagy, and cell migration. This tumor suppressor also enhances retinoblastoma 1 gene expression in cancer cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
UniProt Protein Function:RB1CC1: a putative transcription factor that functions as a key regulator of Rb expression. Required for autophagosome formation. Contains a nuclear localization signal, a leucine zipper motif and a coiled-coil structure. Thought to play a biological role in controlling cell growth and progression of various cancers. Probably involved in the tumorigenesis of breast cancer. It is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. Plays a crucial role in muscular differentiation and required for myogenic differentiation. Part of a complex consisting of ATG13, ULK1 and RB1CC1. This complex associates with ATG101. Under starvation conditions, is localized to phagophores. Expression levels correlated closely with those of RB1 in cancer cell lines as well as in various normal human tissues. Abundantly expressed in human musculoskeletal and cultured osteosarcoma cells. Expression was difficult to detect in immature proliferating chondroblasts or myogenic cells in embryos, but became obvious and prominent concomitantly with the maturation of osteocytes, chondrocytes, and skeletal muscle cells. Expression in these musculoskeletal cells increased with RB1 expression, which is linked to the terminal differentiation of many tissues and cells. The introduction of the wild-type protein decreased the formation of macroscopic colonies in a cell growth assay.
UniProt Protein Details:

Protein type:DNA-binding; Autophagy; Tumor suppressor; Transcription factor

Chromosomal Location of Human Ortholog: 8q11

Cellular Component: nuclear membrane; cytoplasm; pre-autophagosomal structure membrane; cytosol

Molecular Function:protein binding; protein kinase binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of cell size; heart development; JNK cascade; regulation of autophagy; positive regulation of protein amino acid phosphorylation; liver development; cell cycle; autophagic vacuole formation

Disease: Breast Cancer

NCBI Summary:The protein encoded by this gene interacts with signaling pathways to coordinately regulate cell growth, cell proliferation, apoptosis, autophagy, and cell migration. This tumor suppressor also enhances retinoblastoma 1 gene expression in cancer cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2009]
UniProt Code:Q8TDY2
NCBI GenInfo Identifier:160359050
NCBI Gene ID:9821
NCBI Accession:Q8TDY2.3
UniProt Related Accession:Q8TDY2
Molecular Weight:182kDa; 183kDa
NCBI Full Name:RB1-inducible coiled-coil protein 1
NCBI Synonym Full Names:RB1 inducible coiled-coil 1
NCBI Official Symbol:RB1CC1  
NCBI Official Synonym Symbols:CC1; ATG17; FIP200; PPP1R131  
NCBI Protein Information:RB1-inducible coiled-coil protein 1
UniProt Protein Name:RB1-inducible coiled-coil protein 1
UniProt Synonym Protein Names:FAK family kinase-interacting protein of 200 kDa; FIP200
Protein Family:RB1-inducible coiled-coil protein
UniProt Gene Name:RB1CC1  
UniProt Entry Name:RBCC1_HUMAN
Anti-FIP200 Antibody (CAB14685)Western blot analysis of extracts of various cell lines, using FIP200 Rabbit pAb (CAB14685) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-FIP200 Antibody (CAB14685)Western blot analysis of extracts of various cell lines, using FIP200 Rabbit pAb (CAB14685) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-FIP200 Antibody (CAB14685)Immunohistochemistry of paraffin-embedded human esophageal cancer using FIP200 Rabbit pAb (CAB14685) at dilution of 1:100 (40x lens).

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