Anti-RBBP7 Antibody (CAB13456)

Antibody Name:	Anti-RBBP7 Antibody
Antibody SKU:	CAB13456
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP ChIP
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human RBBP7 (NP_002884.1).

Application:	WB IHC IF IP ChIP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 ChIP 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	HT-29, MCF7, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human RBBP7 (NP_002884.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASK EMFE DTVE ERVI NEEY KIWK KNTP FLYD LVMT HALQ WPSL TVQW LPEV TKPE GKDY ALHW LVLG THTS DEQN HLVV ARVH IPND DAQF DASH CDSD KGEF GGFG SVTG KIEC EIKI NHEG EVNR ARYM PQNP HIIA TKTP SSDV LVFD YTKH PAKP DPSG ECNP DLRL RGHQ KEGY GLSW NSNL SGHL LSAS DDHT VCLW DINA GPKE GKIV DAKA IFTG HSAV VE
Gene ID:	5931
Uniprot:	Q16576
Cellular Location:	Nucleus
Calculated MW:	47kDa/52kDa
Observed MW:	48kDa

Synonyms:

RBBP7, RbAp46

Background:

This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	RBBP7: Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. Binds directly to helix 1 of the histone fold of histone H4, a region that is not accessible when H4 is in chromatin. Subunit of the type B histone acetyltransferase (HAT) complex, composed of RBBP7 and HAT1. Subunit of the core histone deacetylase (HDAC) complex, which is composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core HDAC complex associates with SIN3A, ARID4B/SAP180, SAP18, SAP30, SAP130, SDS3/SAP45 and possibly ARID4A/RBP1 and ING1 to form the SIN3 HDAC complex. The core HDAC complex may also associate with MTA2, MBD3, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylase complex (the NuRD complex). The NuRD complex may also interact with MBD3L1 and MBD3L2. Interacts with MTA1. Subunit of the PRC2/EED-EZH2 complex, which is composed of at least EED, EZH2, RBBP4, RBBP7 and SUZ12. The PRC2/EED-EZH2 complex may also associate with HDAC1. Part of the nucleosome remodeling factor (NURF) complex which consists of SMARCA1; BPTF; RBBP4 and RBBP7. Interacts with the viral protein- binding domain of the retinoblastoma protein (RB1). Interacts with CREBBP, and this interaction may be enhanced by the binding of phosphorylated CREB1 to CREBBP. Interacts with BRCA1, HDAC7 and SUV39H1. Belongs to the WD repeat RBAP46/RBAP48/MSI1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: Xp22.2 Cellular Component: ESC/E(Z) complex; nucleoplasm; nucleus; NuRD complex Molecular Function:histone deacetylase activity; protein binding Biological Process: cell proliferation; DNA replication-independent nucleosome assembly at centromere; multicellular organismal development; negative regulation of cell growth; negative regulation of gene expression, epigenetic
NCBI Summary:	This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
UniProt Code:	Q16576
NCBI GenInfo Identifier:	2494891
NCBI Gene ID:	5931
NCBI Accession:	Q16576.1
UniProt Secondary Accession:	Q16576,Q5JP00,
UniProt Related Accession:	Q16576
Molecular Weight:	52,314 Da
NCBI Full Name:	Histone-binding protein RBBP7
NCBI Synonym Full Names:	RB binding protein 7, chromatin remodeling factor
NCBI Official Symbol:	RBBP7Â Â
NCBI Official Synonym Symbols:	RbAp46Â Â
NCBI Protein Information:	histone-binding protein RBBP7
UniProt Protein Name:	Histone-binding protein RBBP7
UniProt Synonym Protein Names:	Histone acetyltransferase type B subunit 2; Nucleosome-remodeling factor subunit RBAP46; Retinoblastoma-binding protein 7; RBBP-7; Retinoblastoma-binding protein p46
Protein Family:	Histone-binding protein
UniProt Gene Name:	RBBP7Â Â
UniProt Entry Name:	RBBP7_HUMAN

	Western blot analysis of extracts of various cell lines, using RBBP7 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
	Western blot analysis of extracts of Rat spleen, using RBBP7 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
	Chromatin immunoprecipitation analysis of extracts of 293T cells, using RBBP7 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Antibodies

Anti-RBBP7 Antibody (CAB6967)

RBBP7 Antibody (PACO11745)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)