Anti-RBM39 Antibody (CAB15760)

Antibody Name:	Anti-RBM39 Antibody
Antibody SKU:	CAB15760
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human RBM39 (NP_001229528.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BxPC-3, U-251MG, 293T, LO2, Mouse brain, Mouse heart

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human RBM39 (NP_001229528.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ATQC FQLS NMFN PQTE EEVG WDTE IKDD VIEE CNKH GGVI HIYV DKNS AQGN VYVK CPSI AAAI AAVN ALHG RWFA GKMI TAAY VPLP TYHN LFPD SMTA TQLL VPSR R
Gene ID:	9584
Uniprot:	Q14498
Cellular Location:	Nucleus speckle
Calculated MW:	57kDa/58kDa/59kDa
Observed MW:	70kDa

Synonyms:

RBM39, CAPER, CAPERalpha, FSAP59, HCC1, RNPC2

Background:

This gene encodes a member of the U2AF65 family of proteins. The encoded protein is found in the nucleus, where it co-localizes with core spliceosomal proteins. It has been shown to play a role in both steroid hormone receptor-mediated transcription and alternative splicing, and it is also a transcriptional coregulator of the viral oncoprotein v-Rel. Multiple transcript variants have been observed for this gene. A related pseudogene has been identified on chromosome X.

UniProt Protein Function:	RNPC2: Transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1. May be involved in pre-mRNA splicing process. Belongs to the splicing factor SR family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding; Nuclear receptor co-regulator; Spliceosome Chromosomal Location of Human Ortholog: 20q11.22 Cellular Component: nucleoplasm; microtubule cytoskeleton; microtubule organizing center; nuclear speck Molecular Function:protein binding; nucleotide binding Biological Process: RNA processing; regulation of transcription, DNA-dependent; transcription, DNA-dependent; RNA splicing; mRNA processing
NCBI Summary:	This gene encodes a member of the U2AF65 family of proteins. The encoded protein is found in the nucleus, where it co-localizes with core spliceosomal proteins. It has been shown to play a role in both steroid hormone receptor-mediated transcription and alternative splicing, and it is also a transcriptional coregulator of the viral oncoprotein v-Rel. Multiple transcript variants have been observed for this gene. A related pseudogene has been identified on chromosome X. [provided by RefSeq, Aug 2011]
UniProt Code:	Q14498
NCBI GenInfo Identifier:	28201880
NCBI Gene ID:	9584
NCBI Accession:	Q14498.2
UniProt Secondary Accession:	Q14498,Q14499, A2RRD3, A5D8W2, B0BLV3, E1P5S0, E1P5S1
UniProt Related Accession:	Q14498
Molecular Weight:	530
NCBI Full Name:	RNA-binding protein 39
NCBI Synonym Full Names:	RNA binding motif protein 39
NCBI Official Symbol:	RBM39
NCBI Official Synonym Symbols:	HCC1; CAPER; RNPC2; FSAP59; CAPERalpha
NCBI Protein Information:	RNA-binding protein 39; CAPER alpha; splicing factor HCC1; hepatocellular carcinoma protein 1; RNA-binding region (RNP1, RRM) containing 2; functional spliceosome-associated protein 59; coactivator of activating protein-1 and estrogen receptors
UniProt Protein Name:	RNA-binding protein 39
UniProt Synonym Protein Names:	CAPER alpha; Hepatocellular carcinoma protein 1; RNA-binding motif protein 39; RNA-binding region-containing protein 2; Splicing factor HCC1
Protein Family:	RNA-binding protein
UniProt Gene Name:	RBM39
UniProt Entry Name:	RBM39_HUMAN

	Western blot analysis of extracts of various cell lines, using RBM39 antibody (CAB15760) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat liver using RBM39 antibody (CAB15760) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using RBM39 antibody (CAB15760) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using RBM39 antibody (CAB15760) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using RBM39 antibody (CAB15760) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

RBM39 Antibody (PACO11759)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)