Recombinant 2019-nCoV Papain-like Protease Protein (His Tag)

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SKU:
RPES0056
€825

Description

Product Background

The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses. Targeting PLpro with antiviral drugs may have an advantage in not only inhibiting viral replication but also inhibiting the dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells.

Bio Activity

Measured by its ability to cleave a fluorogenic peptide substrate, Arg-Leu-Arg-Gly-Gly-AMC (RLRGGAMC).The Specific Activity is >80pmols/min/μg

Key Information Description

Product Name

Recombinant 2019-nCoV Papain-like Protease Protein (His Tag)

Size

  • 100µg
  • 1mg

SKU

RPES0056

Synonyms

Papain-like Protease; PLpro; PL-PRO; pp1a; Replicase polyprotein 1a

Species

Virus

Expression host

E.coli

Sequence

Glu1564-Val1880

Accession

YP_009725299.1

Molar Mass

36.79 kDa

Tag

N-His

Purity

> 90 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Storage

Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Formulation

Lyophilized from sterile 20 mM Tris 500mM NaCl, pH 7.4.

Reconstitution

Please refer to the printed manual for detailed information.

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