The Recombinant 4E-BP1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110441) against 4E-BP1. This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse, Rat and Sheep samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of Jurkat. The target is localized to the nucleus.
Product Name:
Recombinant 4E-BP1 Monoclonal Antibody
SKU:
AGEL5758
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
4E-BP, 4E-BP1, 4EBP, 4EBP1, BP 1, BP-1, eIF4E binding protein, eIF4E binding protein 1, eIF4E-binding protein 1, EIF4EBP, EIF4EBP1, eukaryotic translation initiation factor 4E-binding protein, eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Reactivity:
Human, Mouse, Rat, Sheep
Immunogen:
Recombinant Human 4E-BP1 protein
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110441
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
4E-BP1
Conjugation:
Unconjugated
Observed MW:
17 kDa
Calculated MW:
13 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
Q13541
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.