The Recombinant AID Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C321668) against AID. Activation-induced cytidine deaminase (AID) is thought to modify RNA due to its high homology to the RNA editing enzyme APOBEC-1. This function, however, has not been confirmed in in vitro studies, which show that AID has significant cytidine deaminase activity, and that this activity is blocked by zinc chelation. The B cell immune system must specifically recognize several infectious agents, which vastly outnumber immunoglobulin gene segments present in a given organism. Mechanisms such as somatic hypermutation, isotype switch recombination and gene conversion introduce diversity and specificity to the immune system. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB and IHC applications. It has been validated by WB analysis of K562(negative control), Ramos, Daudi and IHC analysis of Human tonsil, Human diffuse large B-cell lymphoma. The target is localized to the cytoplasm, nucleus.
Product Name:
Recombinant AID Monoclonal Antibody
SKU:
AGEL6973
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
AICDA, AID
Reactivity:
Human
Immunogen:
Peptide. This information is proprietary to PTMab.
Applications:
WBIHC
Clonality:
Recombinant Monoclonal
Clone No:
C321668
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
AID
Conjugation:
Unconjugated
Observed MW:
23 kDa
Calculated MW:
24 kDa
Recommended Dilution:
WB 1:1000; IHC 1:2000
UniProt:
Q9GZX7
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
