The Recombinant AIFM1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110342) against AIFM1. This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of Jurkat. The target is localized to the mitochondrion intermembrane space, Mitochondrion inner membrane. Cytoplasm, nucleus, cytoplasm, perinuclear region, Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis (PubMed:15775970). Colocalizes with EIF3G in the nucleus and perinuclear region (PubMed:17094969), mitochondrion intermembrane space, mitochondrion inner membrane, has a stronger membrane anchorage than isoform 1, mitochondrion, cytoplasm, cytosol, in pro-apoptotic conditions, is released from mitochondria to cytosol in a calpain/cathepsin-dependent manner, cytoplasm.
Product Name:
Recombinant AIFM1 Monoclonal Antibody
SKU:
AGEL5661
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
AIF, AIFM, AIFM1, Apoptosis inducing factor, Apoptosis inducing factor 1, Apoptosis-inducing factor 1, Apoptosis-Inducing Factor 1 Mitochondrial, axonal, axonal motor-sensory, CMT2D, CMTX, CMTX4, COWCK, COXPD, COXPD6, DFNX, DFNX5, Harlequin, mAIF, MGC111425, MGC5706, mitochondrial, mitochondrion associated, motor-sensory with deafness and mental retardation (Cowchock syndrome), NADMR, NAMSD, Neuropathy, PDCD, PDCD 8, PDCD8, Programmed Cell Death 8, Programmed cell death 8 (apoptosis inducing factor), Programmed cell death 8 isoform 1, Programmed cell death 8 isoform 2, Programmed cell death 8 isoform 3, Programmed Cell Death Protein, Programmed Cell Death Protein 8, Programmed cell death protein 8 mitochondrial, Programmed cell death protein 8 mitochondrial precursor, Striatal apoptosis inducing factor, with deafness and mental retardation
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human AIFM1 protein
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110342
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
AIFM1
Conjugation:
Unconjugated
Observed MW:
67 kDa
Calculated MW:
67 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
O95831
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
