The Recombinant ATG4B Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C121361) against ATG4B. ATG4B is a member of the autophagin protein family. Its cysteine protease activity is required for the cytoplasm to vacuole transport (Cvt) and autophagy. ATG4B cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC and FCM applications. It has been validated by WB analysis of 293T, Ramos, PC-12, Mouse brain, Rat brain, IHC analysis of Human colon cancer, Human pancreatic cancer and FCM analysis of Jurkat. The target is localized to the cytoplasm.
Product Name:
Recombinant ATG4B Monoclonal Antibody
SKU:
AGEL6666
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
APG4 autophagy 4 homolog B, APG4B, ATG4 autophagy related 4 homolog B (S. cerevisiae), ATG4B, AUT like 1 cysteine endopeptidase, AUT-like 1 cysteine endopeptidase, AUTL, AUTL1, Autophagin 1, Autophagin-1, Autophagy related 4B cysteine peptidase, Autophagy related cysteine endopeptidase 1, Autophagy related protein 4 homolog B, Autophagy-related cysteine endopeptidase 1, Autophagy-related protein 4 homolog B, cysteine protease ATG4B, hAPG4B, KIAA, KIAA0943, MGC1353
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant human ATG4B fragment
Applications:
WBIHCFCM
Clonality:
Recombinant Monoclonal
Clone No:
C121361
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
ATG4B
Conjugation:
Unconjugated
Observed MW:
44 kDa
Calculated MW:
44 kDa
Recommended Dilution:
WB 1:500-1000; IHC 1:200-1000; FCM 1:50-100
UniProt:
Q9Y4P1
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.