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Recombinant ATG4B Monoclonal Antibody (AGEL6568)

Datasheet MSDS
SKU AGEL6568
Product Type Antibody
Reactivity Human, Mouse, Rat
Applications WB, IF, ELISA
Host Species Rabbit
Clonality Monoclonal
Clone C171263
Conjugate Unconjugated
$606 - $843

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Description

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Recombinant ATG4B Monoclonal Antibody

The Recombinant ATG4B Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C171263) against ATG4B. Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.

This antibody is suitable for WB, IF and ELISA applications. It has been validated by WB analysis of Hela, A375, Mouse kidney, C6. The target is localized to the cytoplasm.

Product Name:Recombinant ATG4B Monoclonal Antibody
SKU:AGEL6568
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:APG4 autophagy 4 homolog B, APG4B, ATG4 autophagy related 4 homolog B (S. cerevisiae), ATG4B, AUT like 1 cysteine endopeptidase, AUT-like 1 cysteine endopeptidase, AUTL, AUTL1, Autophagin 1, Autophagin-1, Autophagy related 4B cysteine peptidase, Autophagy related cysteine endopeptidase 1, Autophagy related protein 4 homolog B, Autophagy-related cysteine endopeptidase 1, Autophagy-related protein 4 homolog B, cysteine protease ATG4B, hAPG4B, KIAA, KIAA0943, MGC1353
Reactivity:Human, Mouse, Rat
Immunogen:The specific immunogen used to produce this antibody is proprietary information.
Applications:WBIFELISA
Clonality:Recombinant Monoclonal
Clone No:C171263
Host:Rabbit
Isotype:Rabbit IgG
Target:ATG4B
Conjugation:Unconjugated
Observed MW:44 kDa
Calculated MW:44 kDa
Recommended Dilution:WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:Q9Y4P1, Q8BGE6
Purification:Protein A
Concentration:1 mg/mL
Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
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