The Recombinant ATG5 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110488) against ATG5. The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of U-87 MG and IHC analysis of Human colon tissue. The target is localized to the cytoplasm.
Product Name:
Recombinant ATG5 Monoclonal Antibody
SKU:
AGEL5803
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
APG, APG 5, APG 5L, APG5, APG5 autophagy 5 like, APG5 like, APG5-LIKE, APG5L, Apoptosis specific protein, Apoptosis-specific protein, ASP, ATG, ATG 5, ATG5, ATG5 autophagy related 5 homolog, Autophagy protein, Autophagy protein 5, Autophagy related 5, hAPG, hAPG5, Homolog of S Cerevisiae autophagy 5, OTTHUMP00000040507
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human ATG5 protein
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110488
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
ATG5
Conjugation:
Unconjugated
Observed MW:
55 kDa
Calculated MW:
33 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
Q9H1Y0
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.