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Recombinant BID Monoclonal Antibody (RMEA0062)

Datasheet MSDS
SKU RMEA0062
Product Type Antibody
Reactivity Human
Applications WB, IHC, ICC/IF, FC, IP
Host Species Rabbit
Clonality Monoclonal
Clone C310048
Conjugate Unconjugated
$540 - $738

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Description

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Recombinant BID Monoclonal Antibody

The Recombinant BID Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C310048) against BID. This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2, and thus regulate apoptosis. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.

This antibody is suitable for WB, IHC, ICC/IF, FC and IP applications. It has been validated by WB analysis of HCT116, Jurkat, 293T, A431, A549, HepG2, IHC analysis of Human colon cancer and IF analysis of HeLa.

Product Name:Recombinant BID Monoclonal Antibody
SKU:RMEA0062
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:BH3-Interacting Domain Death Agonist, BID, FP497, p22 BID
Reactivity:Human
Immunogen:Recombinant Human BID protein
Applications:WBIHCICC/IFFCIP
Clonality:Recombinant Monoclonal
Clone No:C310048
Host:Rabbit
Isotype:Rabbit IgG
Target:BID
Conjugation:Unconjugated
Observed MW:22 kDa
Calculated MW:22 kDa
Recommended Dilution:WB 1:1000; IHC: 1:20; ICC/IF: 1:20; FC: 1:20; IP: 1:20
UniProt:P55957
Purification:Protein A
Concentration:1 mg/mL
Buffer:0.2 µm filtered solution in PBS
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Product ImageImmunofluorescent analysis of HeLa cells (green), and DAPI (blue).
Product ImageImmunohistochemical analysis of paraffin-embedded Human colon cancer tissue sections using BID Antibody (RMEA0062) at a dilution of 1:20 (10× lens). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Product ImageWestern blot analysis of HCT116,Jurkat,293T,A431,A549 and HepG2 cell lysates using BID Antibody (RMEA0062) at a dilution of 1:1000. Observed-MW:22 kDa Calculated-MW:22 kDa
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