The Recombinant Caspase-14/CASP14 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110028) against CASP14. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC-P, ICC/IF, FC and IP applications. It has been validated by WB analysis of MCF7, IHC analysis of Human skin and IF analysis of A431.
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunofluorescence analysis of A431 cells labelling Caspase-14. Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human skin tissue sections using Caspase-14/CASP14 Antibody (AGEL5347) at a dilution of 1:150 (10× lens). Heat mediated antigen retrieval was performed with Tris- EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Western blot analysis of MCF7 cell lysates using Caspase-14/CASP14 Antibody (AGEL5347) at a dilution of 1:1500. Observed-MW:28 kDa Calculated-MW:28 kDa
