The Recombinant CD50/ICAM3 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C341104) against CD50/ICAM3. ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for IHC, IF and FCM applications. It has been validated by IHC analysis of Human colon, Human tonsil, IF analysis of Ramos, Jurkat and FCM analysis of PBMC. The target is localized to the membrane.
Product Name:
Recombinant CD50/ICAM3 Monoclonal Antibody
SKU:
RMEA1105
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
CD50, CD_antigen: CD, CD_antigen: CD50, CDW, CDW50, Cluster of Differentiation, Cluster of Differentiation 50, ICAM, ICAM-3, ICAM-R, ICAM3
Reactivity:
Human
Immunogen:
Recombinant human CD50/ICAM3 fragment
Applications:
IHCIFFCM
Clonality:
Recombinant Monoclonal
Clone No:
C341104
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
CD50/ICAM3
Conjugation:
Unconjugated
Recommended Dilution:
IHC 1:100-500; IF 1:100-200; FCM 1:50-100
UniProt:
P32942
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Flow cytometric analysis of human CD50/ICAM3 expression on PBMC cells. Cells were stained with purified anti-Human CD50/ICAM3, then a Alexa Fluor 488-conjugated second step antibody. The histogram were derived from events with the forward and side light- scatter characteristics of intact cells.
Immunofluorescent analysis of (4% Paraformaldehyde) fixed Ramos cells using anti-CD50/ICAM3 Monoclonal Antibody at dilution of 1:200.
Immunofluorescent analysis of (4% Paraformaldehyde) fixed Jurkat cells using anti-CD50/ICAM3 Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Human colon using CD50/ICAM3 Monoclonal Antibody at dilution of 1:500.
Immunohistochemistry of paraffin-embedded Human tonsil using CD50/ICAM3 Monoclonal Antibody at dilution of 1:1000.
