The Recombinant CD8 beta Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C521603) against CD8 beta. Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Mouse samples.
This antibody is suitable for WB and IHC applications. It has been validated by WB analysis of Mouse lymph (Low expression),?Mouse thymus and IHC analysis of Mouse spleen, Mouse liver (negative tissue). The target is localized to the cell membrane, secreted.
Peptide. This information is proprietary to PTMab.
Applications:
WBIHC
Clonality:
Recombinant Monoclonal
Clone No:
C521603
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
CD8 beta
Conjugation:
Unconjugated
Observed MW:
28-32 kDa
Calculated MW:
24 kDa
Recommended Dilution:
WB 1:1000; IHC 1:100
UniProt:
P10966
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded Mouse spleen using CD8 beta Monoclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded Mouse liver (negative tissue) using CD8 beta Monoclonal Antibody at dilution of 1:100.
Western Blot with CD8 beta Monoclonal Antibody at dilution of 1:1000. Lane 1: Mouse lymph (Low expression), Lane 2: Mouse thymus Observed-MW:28-32 kDa Calculated-MW:24 kDa
