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Recombinant Cdc25C Monoclonal Antibody (AGEL6809)

Datasheet MSDS
SKU AGEL6809
Product Type Antibody
Reactivity Human, Rat, Mouse
Applications WB, IHC, IF, IP, ELISA
Host Species Rabbit
Clonality Monoclonal
Clone C111504
Conjugate Unconjugated
$606 - $843

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Description

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Recombinant Cdc25C Monoclonal Antibody

The Recombinant Cdc25C Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C111504) against Cdc25C. Cdc25C is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis. Cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint. When phosphorylated at Ser216, Cdc25C binds to members of the 14-3-3 family of proteins, sequestering Cdc25C in the cytoplasm and thereby preventing premature mitosis. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.

This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of U-14, Hela, K562 and IHC analysis of Human bladder carcinoma. The target is localized to the nucleus.

Product Name:Recombinant Cdc25C Monoclonal Antibody
SKU:AGEL6809
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:CDC, CDC25, CDC25C, PPP1R, PPP1R60
Reactivity:Human, Mouse, Rat
Immunogen:The specific immunogen used to produce this antibody is proprietary information.
Applications:WBIHCIFIPELISA
Clonality:Recombinant Monoclonal
Clone No:C111504
Host:Rabbit
Isotype:Rabbit IgG
Target:Cdc25C
Conjugation:Unconjugated
Observed MW:58 kDa
Calculated MW:53 kDa
Recommended Dilution:WB 1:2000-10000; IHC 1:1000-4000; IF 1:200-1000; IP 1:50-200; ELISA 1:5000-20000
UniProt:P30307, P48967
Purification:Protein A
Concentration:1 mg/mL
Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
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