The Recombinant CEACAM1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C321041) against CEACAM1. Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner. Plays a role as coinhibitory receptor in immune response, insulin action and functions also as an activator during angiogenesis. Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB, IHC and FCM applications. It has been validated by WB analysis of PC-3 (Negative control), HepG2, IHC analysis of Human colon cancer, Human colon and FCM analysis of PBMCs. The target is localized to the membrane.
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Flow cytometric analysis of human CEACAM1 expression on PBMCs cells. Cells were stained with purified anti- Human CEACAM1, then a Alexa Fluor 488-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunohistochemistry of paraffin-embedded Human colon cancer using CEACAM1 Monoclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded Human colon using CEACAM1 Monoclonal Antibody at dilution of 1:100.
Western Blot with CEACAM1 Monoclonal Antibody at dilution of 1:1000. Lane 1: PC-3 (Negative control), Lane 2: HepG2 Observed-MW:100-180 kDa Calculated-MW:58 kDa
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