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Recombinant Cleaved PARP1 p25 Monoclonal Antibody (RMEA1118)

Datasheet MSDS
SKU RMEA1118
Product Type Antibody
Reactivity Human, Mouse
Applications WB, IHC, IF
Host Species Rabbit
Clonality Monoclonal
Clone C211117
Conjugate Unconjugated
€459 - €639

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Description

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Recombinant Cleaved PARP1 p25 Monoclonal Antibody

The Recombinant Cleaved PARP1 p25 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C211117) against Cleaved PARP1 p25. Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mainly mediates glutamate and aspartate ADP-ribosylation of target proteins: the ADP-D-ribosyl group of NAD+ is transferred to the acceptor carboxyl group of glutamate and aspartate residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human and Mouse samples.

This antibody is suitable for WB, IHC and IF applications. It has been validated by WB analysis of HeLa, Raw264.7, Jurkat, 3T3, IHC analysis of Human breast cancer, Human ovarian cancer and IF analysis of HeLa. The target is localized to the nucleus.

Product Name:Recombinant Cleaved PARP1 p25 Monoclonal Antibody
SKU:RMEA1118
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:ADP-ribosyltransferase diphtheria toxin-like, ADP-ribosyltransferase diphtheria toxin-like 1, ADPRT, ADPRT 1, ADPRT1, APOPAIN, ARTD, ARTD1, Cleaved PARP, NAD(+) ADP-ribosyltransferase, NAD(+) ADP-ribosyltransferase 1, pADPRT, pADPRT-1, PARP, PARP-1, PARP1, Poly [ADP-ribose] polymerase, Poly [ADP-ribose] polymerase 1, Poly ADP ribose polymerase, Poly ADP ribose polymerase 1, poly(ADP-ribose) polymerase, poly(ADP-ribose) polymerase 1, Poly[ADP-ribose] synthase, Poly[ADP-ribose] synthase 1, PPOL, SCA, SCA1
Reactivity:Human, Mouse
Immunogen:Recombinant human Cleaved PARP1 p25 fragment
Applications:WBIHCIF
Clonality:Recombinant Monoclonal
Clone No:C211117
Host:Rabbit
Isotype:Rabbit IgG
Target:Cleaved PARP1 p25
Conjugation:Unconjugated
Observed MW:25 kDa
Calculated MW:25 kDa
Recommended Dilution:WB 1:500-2000; IHC 1:200-1000; IF 1:50-100
UniProt:P09874
Purification:Protein A
Concentration:1 mg/mL
Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Product ImageImmunofluorescent analysis of (100% Ice-cold methanol) fixed HeLa cells using anti-Cleaved PARP1 p25 Monoclonal Antibody at dilution of 1:100.
Product ImageImmunohistochemistry of paraffin-embedded Human breast cancer using Cleaved PARP1 p25 Monoclonal Antibody at dilution of 1:1000.
Product ImageImmunohistochemistry of paraffin-embedded Human ovarian cancer using Cleaved PARP1 p25 Monoclonal Antibody at dilution of 1:1000.
Product ImageWestern Blot with Cleaved PARP1 p25 Monoclonal Antibody at dilution of 1:2000. Lane 1: HeLa, Lane 2: Raw264.7, Lane 3: Jurkat, Lane 4: 3T3 Observed-MW:25 kDa Calculated-MW:25 kDa
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