The Recombinant Collagen IV Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C310730) against Collagen IV. This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of Hela and IHC analysis of Human appendix tissue. The target is localized to the cytoplasmic.
IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P02462
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded human appendix tissue using Recombinant Collagen IV Monoclonal Antibody at dilution of 1:200.
Western Blot with Recombinant Collagen IV Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: Hela cells. Observed-MW:200 kDa Calculated-MW:160 kDa
