The Recombinant CTCF Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110333) against CTCF. This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of HEK293. The target is localized to the nucleus.
Product Name:
Recombinant CTCF Monoclonal Antibody
SKU:
AGEL5652
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
11-Zinc Finger Protein, BORIS, Brother of the regulator of imprinted sites, Cancer/testis antigen, Cancer/testis antigen 27, CCCTC-Binding Factor, CT27, CTCF, CTCF paralog, CTCF-like protein, CTCFL, CTCFL Paralog, MRD, MRD21, Transcriptional Repressor CTCF, Transcriptional repressor CTCFL, Zinc finger protein CTCF-T
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human CTCF protein
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110333
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
CTCF
Conjugation:
Unconjugated
Observed MW:
140 kDa
Calculated MW:
83 kDa
Recommended Dilution:
IHC 1:2000-10000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P49711
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.