The Recombinant DDX4 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110920) against DDX4. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of Mouse testis. The target is localized to the cytoplasm, cytoplasm, perinuclear region, component of the meiotic nuage, also named p granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.
Product Name:
Recombinant DDX4 Monoclonal Antibody
SKU:
AGEL6226
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
DDX, DDX 4, DDX4, DEAD (Asp Glu Ala Asp) box polypeptide, DEAD (Asp Glu Ala Asp) box polypeptide 4, DEAD box protein, DEAD box protein 4, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4, MVH, Probable ATP dependent RNA helicase DDX, Probable ATP dependent RNA helicase DDX4, Probable ATP-dependent RNA helicase DDX, Probable ATP-dependent RNA helicase DDX4, VASA, Vasa homolog
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human DDX4 protein
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110920
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
DDX4
Conjugation:
Unconjugated
Observed MW:
79 kDa
Calculated MW:
79 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
Q9NQI0
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.