The Recombinant DNA PKcs Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110446) against DNA PKcs. This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of HT-29. The target is localized to the nucleus.
Product Name:
Recombinant DNA PKcs Monoclonal Antibody
SKU:
AGEL5763
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
complementing 1, DNA dependent protein kinase catalytic subunit, DNA PK catalytic subunit, DNA-dependent protein kinase catalytic subunit, DNA-PK catalytic subunit, DNA-PKcs, DNAPK, DNAPK catalytic subunit, DNPK, DNPK 1, DNPK1, homolog of 1, Hyper radiosensitivity of murine scid mutation, Hyperradiosensitivity complementing 1, HYRC, HYRC 1, HYRC1, IMD, IMD26, mouse, p350, p460, PKRDC, PRKDC, Protein Kinase DNA Activated Catalytic Polypeptide, XRCC, XRCC 7, XRCC7
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human DNA PKcs protein
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110446
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
DNA PKcs
Conjugation:
Unconjugated
Observed MW:
469 kDa
Calculated MW:
469 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P78527
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant DNA PKcs Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: HT-29 whole cell lysate. Observed-MW:469 kDa Calculated-MW:469 kDa