The Recombinant PKC mu Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C331606) against PKC mu. Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction.PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB applications. It has been validated by WB analysis of HeLa,?PC-3, LNCaP. The target is localized to the cell membrane, Golgi apparatus, cytoplasm.
Product Name:
Recombinant PKC mu Monoclonal Antibody
SKU:
AGEL6911
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
CHDED, KPCD1, nPKC D1, nPKC mu, nPKC-D1, nPKC-mu, nPKCD1, nPKCmu, PKC, PKC MU, PKC-MU, PKCM, PKCmu, PKD, PKD 1, PKD1, PRKCM, PRKD, PRKD 1, PRKD1, Protein kinase C mu, Protein kinase C mu type, Protein kinase D, Protein kinase D1, Serine/threonine protein kinase D1, Serine/threonine-protein kinase D1
Reactivity:
Human
Immunogen:
Peptide. This information is proprietary to PTMab.
Applications:
WB
Clonality:
Recombinant Monoclonal
Clone No:
C331606
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
PKC mu
Conjugation:
Unconjugated
Observed MW:
110 kDa
Calculated MW:
102 kDa
Recommended Dilution:
WB 1:1000
UniProt:
Q15139
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with PKC mu Monoclonal Antibody at dilution of 1:1000. Lane 1: HeLa, Lane 2: PC-3, Lane 3: LNCaP Observed-MW:110 kDa Calculated-MW:102 kDa
