The Recombinant EED Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C231656) against EED. The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death and cell-cycle arrest. PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human and Mouse samples.
This antibody is suitable for WB applications. It has been validated by WB analysis of HepG2,?THP-1, NIH-3T3. The target is localized to the nucleus.
Peptide. This information is proprietary to PTMab.
Applications:
WB
Clonality:
Recombinant Monoclonal
Clone No:
C231656
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
EED
Conjugation:
Unconjugated
Observed MW:
50-70 kDa
Calculated MW:
50 kDa
Recommended Dilution:
WB 1:1000
UniProt:
O75530
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with EED Monoclonal Antibody at dilution of 1:1000. Lane 1: HepG2, Lane 2: THP-1, Lane 3: NIH-3T3 Observed-MW:50-70 kDa Calculated-MW:50 kDa
