The Recombinant eIF4A2 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C121689) against eIF4A2. Eukaryotic initiation factor 4A (eIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesisbegins, as eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the smallribosomal subunit, and subsequent scanning for the initiator codon. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC and IP applications. It has been validated by WB analysis of 293T,?MCF-7, HeLa, RAW264.7, PC-12, IHC analysis of Human cerebrum, Human breast cancer, Mouse liver, Rat cardiac muscle and IP analysis of 293T cells extracts. The target is localized to the nucleus.
