The Recombinant Estrogen Receptor α Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110358) against Estrogen Receptor α. This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of MCF7. The target is localized to the nucleus.
7*/654 isoform, 7*/819 2 isoform, 7*/822 isoform, 8*/901 isoform, 8*/941 isoform, DKFZp686N23123, ER, ER alpha, ER-Alpha, Era, ESR, ESR1, ESRA, Estradiol Receptor, Estrogen nuclear receptor alpha, Estrogen Receptor, Estrogen receptor 1, Estrogen receptor alpha, Estrogen receptor alpha 3*, Estrogen receptor alpha delta 3*, Estrogen receptor alpha delta 4 +49 isoform, Estrogen receptor alpha E1 E2 1 2, Estrogen receptor alpha E1 N2 E2 1 2, Estrogen Receptor α, ESTRR, group A, member, member 1, NR3A, NR3A1, nuclear receptor subfamily, nuclear receptor subfamily 3, Nuclear Receptor Subfamily 3 Group A Member, Nuclear Receptor Subfamily 3 Group A Member 1
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human Estrogen Receptor α protein
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110358
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Estrogen Receptor α
Conjugation:
Unconjugated
Observed MW:
66 kDa
Calculated MW:
66 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P03372
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant Estrogen Receptor α Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: MCF7 whole cell lysate. Observed-MW:66 kDa Calculated-MW:66 kDa
