The Recombinant FGL1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C321542) against FGL1. Fibrinogen-like protein 1 (FGL1), also known as hepassocin, is a secreted protein mainly expressed in liver and its expression is further enhanced following acute liver injury. FGL1 null mice have multiple metabolic abnormalities and are more prone to develop hepatocellular carcinoma under certain experimental conditions, suggesting FGL1 has diverse functions in vivo. More recently, FGL1 was found to be highly expressed in human cancer cells and function as an inhibitory ligand for LAG3. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB, IHC-P, FC and IP applications. It has been validated by WB analysis of HepG2, Huh1. The target is localized to the secreted.
Product Name:
Recombinant FGL1 Monoclonal Antibody
SKU:
AGEL6847
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
FGL, FGL 1, FGL1, Fibrinogen like 1, Fibrinogen like protein 1, Fibrinogen related protein 1, Fibrinogen-like protein, Fibrinogen-like protein 1, Hepassocin, Hepatocellular carcinoma related sequence, Hepatocyte derived fibrinogen related protein 1, Hepatocyte-derived fibrinogen-related protein 1, HFREP 1, HFREP-1, HFREP1, HP 041, HP-041, HP041, HPS, LFIRE, LFIRE 1, LFIRE-1, LFIRE1, Liver fibrinogen related protein 1, Liver fibrinogen-related protein, Liver fibrinogen-related protein 1, MFIRE 1, MGC108569, MGC12455, MGC37822, OTTHUMP00000122468
Reactivity:
Human
Immunogen:
Recombinant protein of human FGL1
Applications:
WBIHC-PFCIP
Clonality:
Recombinant Monoclonal
Clone No:
C321542
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
FGL1
Conjugation:
Unconjugated
Observed MW:
36 kDa
Calculated MW:
36 kDa
Recommended Dilution:
WB 1:1000; IHC-P 1:200-500; IP 1:20-50; FCM 1:20-50
UniProt:
Q08830
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western blot analysis of HepG2 cell lysates using FGL1 Antibody (AGEL6847) at a dilution of 1:1000. Observed-MW:36 kDa Calculated-MW:36 kDa
Western blot analysis of Huh1 cell lysates using FGL1 Antibody (AGEL6847) at a dilution of 1:1000. Observed-MW:36 kDa Calculated-MW:36 kDa