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Key Features

Recombinant GPA33 Monoclonal Antibody (AGEL5407)

Datasheet MSDS
SKU AGEL5407
Product Type Antibody
Reactivity Human
Applications WB, IHC-P, IP
Host Species Rabbit
Clonality Monoclonal
Clone C320088
Conjugate Unconjugated
$540 - $738

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Description

system_update_altDatasheetsystem_update_altMSDS

Recombinant GPA33 Monoclonal Antibody

The Recombinant GPA33 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C320088) against GPA33. The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.

This antibody is suitable for WB, IHC-P and IP applications. It has been validated by WB analysis of HT-29 and IHC analysis of Human colon.

Product Name:Recombinant GPA33 Monoclonal Antibody
SKU:AGEL5407
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:A33, Cell surface A33 antigen, Cell surface A33 antigen precursor, Glycoprotein A, Glycoprotein A33, Glycoprotein A33 (transmembrane), GPA, GPA33, MGC129986, MGC129987, OTTHUMP, OTTHUMP00000032346, Transmembrane glycoprotein A, Transmembrane glycoprotein A33
Reactivity:Human
Immunogen:Recombinant Human GPA33 protein
Applications:WBIHC-PIP
Clonality:Recombinant Monoclonal
Clone No:C320088
Host:Rabbit
Isotype:Rabbit IgG
Target:GPA33
Conjugation:Unconjugated
Observed MW:45 kDa
Calculated MW:36 kDa
Recommended Dilution:WB 1:1000; IHC-P: 1:100-200; IP: 1:20
UniProt:Q99795
Purification:Protein A
Concentration:1 mg/mL
Buffer:0.2 µm filtered solution in PBS
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Product ImageImmunohistochemical analysis of paraffin-embedded Human colon tissue sections using GPA33 Antibody (AGEL5407) at a dilution of 1:150 (10× lens). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Product ImageWestern blot analysis of HT-29 cell lysates using GPA33 Antibody (AGEL5407) at a dilution of 1:1000. Observed-MW:45 kDa Calculated-MW:36 kDa
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