The Recombinant HMGB1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C121177) against HMGB1. Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, FCM and IP applications. It has been validated by WB analysis of HeLa, HepG2, Jurkat, NIH/3T3, PC-12, IHC analysis of Human kidney, Mouse stomach, Rat colon, FCM analysis of HeLa and IP analysis of Jurkat cells extracts. The target is localized to the nucleus.
Product Name:
Recombinant HMGB1 Monoclonal Antibody
SKU:
AGEL6483
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
Amphoterin, Chromosomal protein, DKFZp686A04236, High mobility group 1, High mobility group box 1, High Mobility Group Protein, High Mobility Group Protein 1, High mobility group protein 1 (HMG-1), High mobility group protein B, High mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, high-mobility group box 1, HMG, HMG-1, HMG1, HMG1DKFZp686A04236, HMG3, HMGB, HMGB 1, HMGB1, nonhistone, NONHISTONE CHROMOSOMAL PROTEIN HMG1, SBP, SBP 1, SBP-1, Sulfoglucuronyl carbohydrate binding protein
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant human HMGB1 fragment
Applications:
WBIHCFCMIP
Clonality:
Recombinant Monoclonal
Clone No:
C121177
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
HMGB1
Conjugation:
Unconjugated
Observed MW:
25 kDa
Calculated MW:
25 kDa
Recommended Dilution:
WB 1:500-2000; IHC 1:200-1000; FCM 1:50-100; IP 1:25
UniProt:
P09429
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
