The Recombinant HMGB1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110484) against HMGB1. HMGB1(high mobility group box 1) Homo sapiens. This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of Hela and IHC analysis of Mouse liver. The target is localized to the nucleus.
Product Name:
Recombinant HMGB1 Monoclonal Antibody
SKU:
AGEL5799
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
Amphoterin, Chromosomal protein, DKFZp686A04236, High mobility group 1, High mobility group box 1, High Mobility Group Protein, High Mobility Group Protein 1, High mobility group protein 1 (HMG-1), High mobility group protein B, High mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, high-mobility group box 1, HMG, HMG-1, HMG1, HMG1DKFZp686A04236, HMG3, HMGB, HMGB 1, HMGB1, nonhistone, NONHISTONE CHROMOSOMAL PROTEIN HMG1, SBP, SBP 1, SBP-1, Sulfoglucuronyl carbohydrate binding protein
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human HMGB1 protein
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110484
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
HMGB1
Conjugation:
Unconjugated
Observed MW:
25 kDa
Calculated MW:
25 kDa
Recommended Dilution:
IHC 1:2000-10000; WB 1:5000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P09429
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded mouse liver using Recombinant HMGB1 Monoclonal Antibody at dilution of 1:200.
Western Blot with Recombinant HMGB1 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: Hela cells. Observed-MW:25 kDa Calculated-MW:25 kDa