Recombinant Human ALDH1A1/ALDC Protein (His Tag) (RPES2227)

SKU:
RPES2227
€289

Description

ELISA Kit Technical Manual

Recombinant Human ALDH1A1/ALDC Protein (His Tag)

Background:Aldehyde Dehydrogenase Family 1 Member A1 (ALDH1A1) is a cytoplasmic enzyme that belongs to the Aldehyde Dehydrogenase family. ALDH1A1 is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties and subcellular localizations. ALDH1A1 is the main cytosolic isoform, which has a lower affinity for aldehydes than the mitochondrial enzyme. ALDH1A1 binds free retinal and cellular retinol-binding protein-bound retinal. It can convert/oxidize retinaldehyde to retinoic acid.
Name:Recombinant Human ALDH1A1/ALDC Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES2227
Synonyms:Retinal Dehydrogenase 1; RALDH 1; RalDH1; ALDH-E1; ALHDII; Aldehyde Dehydrogenase Family 1 Member A1; Aldehyde Dehydrogenase Cytosolic; ALDH1A1; ALDC; ALDH1; PUMB10;ALDH11;HEL-9;HEL-S-53e;HEL12;PUMB1;RALDH1
Species:Human
Expression host:E.coli
Sequence:Met 1-Ser501
Accession:P00352
Mol Mass:57.0 kDa
AP Mol Mass:60 kDa
Tag:N-6His
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Formulation:Supplied as a 0.2 um filtered solution of 20mM TrisHCl?pH8.5.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:ALDH1A1: Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. Belongs to the aldehyde dehydrogenase family.
UniProt Protein Details:

Protein type:GAPs, Ras; EC 1.2.1.36; GAPs; Cofactor and Vitamin Metabolism - retinol; Apoptosis; Oxidoreductase

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: cytoplasm; cytosol

Molecular Function:aldehyde dehydrogenase (NAD) activity; androgen binding; 3-chloroallyl aldehyde dehydrogenase activity; benzaldehyde dehydrogenase (NAD+) activity; retinal dehydrogenase activity

Biological Process: retinol metabolic process; xenobiotic metabolic process; aldehyde metabolic process; ethanol oxidation

NCBI Summary:The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]
UniProt Code:P00352
NCBI GenInfo Identifier:118495
NCBI Gene ID:216
NCBI Accession:P00352.2
UniProt Related Accession:P00352
Molecular Weight:~ 50-55kDa
NCBI Full Name:Retinal dehydrogenase 1
NCBI Synonym Full Names:aldehyde dehydrogenase 1 family member A1
NCBI Official Symbol:ALDH1A1  
NCBI Official Synonym Symbols:ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e  
NCBI Protein Information:retinal dehydrogenase 1
UniProt Protein Name:Retinal dehydrogenase 1
UniProt Synonym Protein Names:ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic
Protein Family:Retinal dehydrogenase
UniProt Gene Name:ALDH1A1  
UniProt Entry Name:AL1A1_HUMAN
View AllClose

Additional Information

Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
View AllClose