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Human AMPK (G1/B2/A1) Heterotrimer Recombinant Protein (Active) (RPES0614)

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SKU:
RPES0614
Product Type:
Recombinant Protein
Species:
Human
Research Area:
Metabolism
€1,949

Description

system_update_altDatasheet
Product Name:AMPK (G1/B2/A1)Recombinant Protein (RPES0614)
Product Code:RPES0614
Size:50 µg
Species:Human
Expressed Host:Baculovirus-Insect Cells
Synonyms:AMPK,AMPKa1
Accession:P54619
Sequence:Met 1-Pro 331
Fusion tag:
Activity:The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
Endotoxin:<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331); constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272); constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus; constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
Purity:> 94 % as determined by reducing SDS-PAGE.
Mol Mass:160 (38+30+92) kDa
AP Mol Mass:35; 37 & 95 kDa
Formulation:Supplied as sterile 50mM Tris; 200mM NaCl; 2mM GSH; 1mM EDTA; 1mM DTT; 10% gly; pH 7.4
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Stability and Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

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