Apurinic-Apyrimidinic Endonuclease 1 (APE1) is required for efficient DNA base excision repair. When the damaged bases in your DNA are removed by the DNA glycosylase; APE1 cuts the AP site to allow resynthesis and ligation to finish the repair. APE1 stimulates the DNA binding activity of many transcription factors; which participate in cancer promotion and progression. APE1 controls the redox status of several transcription factors, such as c-Jun; c-Fos; NF-kB; and p53. APEN is also involved in calcium-dependent down-regulation of PTH expression. Human APE1/APE Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Recombinant Human Apurinic-Apyrimidinic Endonuclease 1 is produced by our E.coli expression system and the target gene encoding Pro2-Leu318 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
35.6 kDa
AP Mol Mass:
40 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 10mM HEPES; 100mM KCl; 50% Glycerol; pH 7.4.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
