Recombinant Human APOM Protein (Fc Tag) (RPES4977)

SKU:
RPES4977
€227

Description

ELISA Kit Technical Manual

Recombinant Human APOM Protein (Fc Tag)

Background:ApoM (apolipoprotein M) is an apolipoprotein and member of the lipocalin protein family. The lipocalins share limited regions of sequence homology and a common tertiary structure architecture. They have an eight-stranded, antiparallel, symmetrical _-barrel fold, which is in essence a beta sheet which has been rolled into a cylindrical shape. Inside this barrel is located a ligand binding site. They transport small hydrophobic molecules such as steroids, bilins, retinoids, and lipids. Lipocalins have been associated with many biological processes, among them immune response, pheromone transport, biological prostaglandin synthesis, retinoid binding, and cancer cell interactions. Lipocalins are comparatively small in size, and are thus less complicated to study as opposed to large, bulky proteins. They can also bind to various ligands for different biological purposes. ApoM is associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. ApoM is involved in lipid transport and can bind sphingosine-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
Name:Recombinant Human APOM Protein (Fc Tag)
Size:20µg
Assay Genie SKU:RPES4977
Synonyms:Apolipoprotein M; Apo-M; ApoM; Protein G3a; APOM; G3A; NG20
Species:Human
Expression host:HEK293 Cells
Sequence:Met 1-Asn 188
Accession:O95445
Mol Mass:45.6 kDa
AP Mol Mass:50 kDa
Tag:C-Fc
Purity:> 85 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from sterile PBS, pH 7.4
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:APOM: Probably involved in lipid transport. Can bind sphingosine-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid. Plasma protein. Expressed in liver and kidney. Belongs to the calycin superfamily. Lipocalin family. Highly divergent.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: integral to plasma membrane

Molecular Function:antioxidant activity; lipid transporter activity; phospholipid binding

Biological Process: cholesterol homeostasis; reverse cholesterol transport; response to glucose stimulus; cholesterol efflux; lipoprotein metabolic process

NCBI Summary:The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Alternate splicing results in both coding and non-coding variants of this gene. [provided by RefSeq, Jan 2012]
UniProt Code:O95445
NCBI GenInfo Identifier:17370872
NCBI Gene ID:55937
NCBI Accession:O95445.2
UniProt Secondary Accession:O95445,Q5SRP4, Q9P046, Q9UMP6, B0UX98,
UniProt Related Accession:O95445
Molecular Weight:188
NCBI Full Name:Apolipoprotein M
NCBI Synonym Full Names:apolipoprotein M
NCBI Official Symbol:APOM
NCBI Official Synonym Symbols:G3a; NG20; apo-M; HSPC336
NCBI Protein Information:apolipoprotein M; protein G3a; NG20-like protein; alternative name: G3a, NG20
UniProt Protein Name:Apolipoprotein M
UniProt Synonym Protein Names:Protein G3a
Protein Family:Apomucin
UniProt Gene Name:APOM
UniProt Entry Name:APOM_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
HEK293 Cells
Reactivity:
Human
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