ATG5 is an E2 ubiquitin ligase that is involved in autophagy. Its expression occurs at the end of the apoptotic process, following on from caspase activity, and is highly expressed in apoptotic cells. It is generated by the action of ATG7, binds to and associates with ATG12 on the isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane soon before or after autophagosome formation is finished. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Human ATG5 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human ATG5 Recombinant Protein (RPES3181)
Product Code:
RPES3181
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Autophagy protein 5, APG5-like, Apoptosis-specific protein, APG5L, ASP, ATG5,
Accession:
Q9H1Y0
Sequence:
Met 1-Asp275
Fusion tag:
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Autophagy Related 5 Homolog is produced by our E.coli expression system and the target gene encoding Met1-Asp275 is expressed.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
32.4 kDa
AP Mol Mass:
33 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM Tris,0.2M NaCl,1mM DTT,40%glycerol, pH 8.0 .
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
