The precursor form of nerve growth factor (proNGF) is similarly afflicted by the cystin knot motif that includes three cystine bridges, whereas proneurotrophins and mature neurotrophins produce different biological responses. ProNGF functions preferentially via the complex of pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin inducing neuronal apoptosis and contributing to age- and disease-related neurodegeneration. Human Beta-NGF/NGFB Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Beta-NGF/NGFB Recombinant Protein (RPES0265)
Product Code:
RPES0265
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB
Accession:
P01138
Sequence:
Glu19-Ala241
Activity:
Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 32697 pg/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human pro-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Glu19-Ala241 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
25 kDa
AP Mol Mass:
30 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 250mM NaCl; pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
