NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF. NGF signaling has been shown to play an important role in neuroprotection and repair. β-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems.
Product Name:
Mouse beta-NGF/NGFB Recombinant Protein (RPES3849)
Product Code:
RPES3849
Size:
10µg
Species:
Mouse
Expressed Host:
E.coli
Synonyms:
Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB
Accession:
P01139
Sequence:
Met130-Arg239
Activity:
Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 0.5-1.5 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse beta-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Met130-Arg239 is expressed.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
12.4 kDa
AP Mol Mass:
12 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris,200mM NaCl,pH8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
